Coding

Part:BBa_K220002:Design

Designed by: Sarah Sandock   Group: iGEM09_Wisconsin-Madison   (2009-10-18)

Glycine Sarcosine Methyl Transferase Gene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 843
    Illegal XbaI site found at 834
    Illegal SpeI site found at 16
    Illegal PstI site found at 7
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 843
    Illegal NheI site found at 556
    Illegal SpeI site found at 16
    Illegal PstI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 843
    Illegal BamHI site found at 330
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 843
    Illegal XbaI site found at 834
    Illegal SpeI site found at 16
    Illegal PstI site found at 7
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 843
    Illegal XbaI site found at 834
    Illegal SpeI site found at 16
    Illegal PstI site found at 7
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization and synthesis of its coding region from Ectothiorhodospira Halochloris


Source

Codon optomization and synthesis of its coding region from Ectothiorhodospira Halochloris


References

A Nyyssölä, T Reinikainen, M Leisola Characterization of Glycine Sarcosine N-Methyltransferase and Sarcosine Dimethylglycine N-Methyltransferase http://www.ncbi.nlm.nih.gov/pmc/articles/PMC92834/?tool=pubmed